pc3 cell lines  (ATCC)

Journal: Cell Reports Methods

Article Title: Biobank of genetically defined murine prostate cancer tumoroids uncovers oncogenic pathways and drug vulnerabilities driven by PTEN-loss

doi: 10.1016/j.crmeth.2026.101370

Figure Lengend Snippet: The PDPK1/AKT/FLT DPI and tenovin-6 (T6) show high anti-cancer efficacy in murine tumoroids and human PCa cell lines (A) Dose-response curves for DPI (top) and T6 (bottom) for in vivo and in vitro Pten KO (left), Pten/Stat3 KO (middle), and Pten/Tp53 KO (right) tumoroids. Points represent means of technical duplicates per tumoroid line ( N = 3). Curve fitting was performed using GraphPad Prism 8.0.2. (B) Bar graphs showing means and ±SD of half-maximal inhibitory concentration (IC50) for DPI (top) and T6 (bottom) for in vivo and in vitro tumoroid lines of all genotypes ( N = 3). Statistical analysis was performed using GraphPad Prism 8.0.2 (one-way ANOVA, Tukey’s test). p > 0.05 if not specified otherwise, ∗ p ≤ 0.05. (C) Bar graphs depicting means and ±SD of IC50 values of DPI (left) and T6 (right) on human PCa cell lines. 22RV1: primary PCa; LNCaP: metastatic PCa; DU145, PC3: metastatic castration-resistant PCa ( N = 3). Statistical analysis was performed using GraphPad Prism 8.0.2 (one-way ANOVA). p > 0.05 if not specified otherwise, ∗ p ≤ 0.05; ∗∗ p ≤ 0.01. (D) Heatmaps of synergy scores calculated with the highest single agent (HSA) model for DPI and enzalutamide (left), and T6 and enzalutamide (right) on the human LNCaP cell line. Values > 0 represent synergistic effects, and values < 0 represent antagonistic effects. IC50 concentrations of respective compounds are underlined ( N = 3). See also .

Article Snippet: Human PC3 metastatic PCa cell line , ATCC , CRL-1435; RRID:CVCL_0035.

Techniques: In Vivo, In Vitro, Concentration Assay

Journal: Journal of Toxicology

Article Title: Phillyrin Modulates AMPK‐Associated Cellular Responses and Migration in PC3 Prostate Cancer Cells

doi: 10.1155/jt/2022957

Figure Lengend Snippet: PHN activates mitochondrial biogenesis. PHN attenuates mitochondrial dysfunction of PC3 cells. The ratio of COX1/SDH‐A was utilized to indicate mitochondrial biogenesis. PHN attenuates mitochondrial dysfunction in PC3 cells.

Article Snippet: The PC3 cell line used in this study was obtained from the American Type Culture Collection (Rockville, MD, USA).

Techniques:

Journal: bioRxiv

Article Title: Dynamic optimization of extrachromosomal DNA copy number drives tumour evolution

doi: 10.64898/2026.03.20.713026

Figure Lengend Snippet: (a) Schematics depicting hypothesized ecDNA copy number distributions of all cells, G1 cells, and parent cells giving rise to daughter cells (left). Representative images from combined IF for Aurora B Kinase and Cyclin A and DNA FISH for MYC in COLO 320DM. Cyclin A was used as a marker to identify G1 interphase cells, whose copy number should theoretically be 1n before entering S-phase and undergoing genome doubling. Aurora B Kinase was used as an identification marker for recently divided daughter cells. Copy number of parents of daughter cells was estimated by taking the average of the daughter cells’ MYC DNA FISH foci areas. Copy numbers of parent cells were compared against copy numbers of G1 interphase cells to avoid high copy number bias arising from cells past S-phase and with replicated DNA content. Scale bars, 10 µm (right). (b) Histograms depicting proportion of G1 interphase and parent cells in each copy number quintile in COLO 320DM and PC3 DM.

Article Snippet: COLO 320DM and COLO 320HSR (colorectal cancer) and the parental PC3 (prostate cancer) cell lines were obtained from ATCC.

Techniques: Marker